pLV2-CMV-EGFP-Ezh2-11-Puro pla

pLV2-CMV-EGFP-Ezh2-11-Puro plasmid

PVT86973

pLV2-CMV-EGFP-Ezh2-11-Puro plasmid Information

Plasmid name: pLV2-CMV-EGFP-Ezh2(mouse)-11-Puro
Alias: NM_001409818.1;Enx-1; Enx1h; KMT6; mKIAA4065
Gene lenght: 2214bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLVX-EGFP-Puro
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-EGFP-Ezh2-11-Puro plasmid Description

pLV2-CMV-EGFP-Ezh2-11-Puro This is a plasmid suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate the transcription of the mouse Ezh2 gene. The plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating the stable integration of the gene into host cells. The plasmid expresses EGFP green fluorescent protein fused at the N-terminus of the Ezh2 gene, allowing for the detection of the expression and localization of the target gene. The plasmid carries a Puro resistance gene for the selection of host cells expressing the target gene.

pLV2-CMV-EGFP-Ezh2-11-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt 2881 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 2941 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 3001 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 3061 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 3121 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 3181 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 3241 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 3301 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 3361 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 3421 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 3481 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 3541 catggacgag ctgtacaagt ccggactcag atctcgagGC GGCGGAGGGA GTGGAGGAGG 3601 GGGAAGCgga ggcggaggga gtggatccat gggccagact gggaagaaat ctgagaaggg 3661 accggtttgt tggcggaagc gtgtaaaatc agagtacatg agactgagac agctcaagag 3721 gttcagaaga gctgatgaag taaagactat gtttagttcc aatcgtcaga aaattttgga 3781 aagaactgaa accttaaacc aagagtggaa gcagcggagg atacagcctg tgcacatcat 3841 gacttcttgt tcagtcacca gtgacttgga ttttccagca caagtcatcc cgttaaagac 3901 cctgaatgca gtcgcctcgg tgcctataat gtactcttgg tcgcccttac aacagaattt 3961 tatggtggaa gacgaaactg ttttacataa cattccttat atgggggatg aagttctgga 4021 tcaggatggc actttcattg aagaactaat aaaaaattat gatggaaaag tgcatggtga 4081 cagagaatgt ggatttataa atgatgaaat ttttgtggag ttggtaaatg ctcttggtca 4141 atataatgat gatgatgatg acgatgatgg agatgatcca gatgaaagag aagaaaaaca 4201 gaaagatcta gaggataatc gagatgataa agaaacttgc ccacctcgga aatttcctgc 4261 tgataaaata tttgaagcca tttcctcaat gtttccagat aagggcaccg cagaagaact 4321 gaaagaaaaa tataaagaac tcacggagca gcagctccca ggtgctctgc ctcctgaatg 4381 tactccaaac atcgatggac caaatgccaa atctgttcag agggagcaaa gcttgcattc 4441 atttcatacg ctcttctgtc gacgatgttt taagtatgac tgcttcctac atcccttcca 4501 tgcaacaccc aacacatata agaggaagaa cacagaaaca gctttggaca acaagccttg 4561 tggaccacag tgttaccagc atctggaggg agctaaggag tttgctgctg ctcttactgc 4621 tgagcgtata aagacaccac ctaaacgccc agggggccgc agaagaggaa gacttccgaa 4681 taacagtagc agacccagca cccccaccat cagtgtgctg gagtcaaagg atacagacag 4741 tgacagagaa gcagggactg aaactggggg agagaacaat gataaagaag aagaagagaa 4801 aaaagatgag acgtccagct cctctgaagc aaattctcgg tgtcaaacac caataaagat 4861 gaagccaaat attgaacctc ctgagaatgt ggagtggagt ggtgctgaag cctccatgtt 4921 tagagtcctc attggtactt actacgataa cttttgtgcc attgctaggc taattgggac 4981 caaaacatgt agacaggtgt atgagtttag agtcaaggag tccagtatca tagcacctgt 5041 tcccactgag gatgtagaca ctcctccaag aaagaagaaa aggaaacatc ggttgtgggc 5101 tgcacactgc agaaagatac aactgaaaaa ggacggctcc tctaaccatg tttacaacta 5161 tcaaccctgt gaccatccac ggcagccttg tgacagttcg tgcccttgtg tgatagcaca 5221 aaatttttgt gaaaagtttt gtcaatgtag ttcagagtgt caaaaccgct ttcctggatg 5281 tcggtgcaaa gcacaatgca acaccaaaca gtgtccatgc tacctggctg tccgagagtg 5341 tgaccctgac ctctgtctca cgtgtggagc tgctgaccat tgggacagta aaaatgtatc 5401 ctgtaagaac tgtagcattc agcggggctc taaaaagcac ttactgctgg caccgtctga 5461 tgtggcaggc tggggcatct ttatcaaaga tcctgtacag aaaaatgaat tcatctcaga 5521 atactgtggg gagattattt ctcaggatga agcagacaga agaggaaaag tgtatgacaa 5581 atacatgtgc agctttctgt tcaacttgaa caatgatttt gtggtggatg caacccgaaa 5641 gggcaacaaa attcgttttg ctaatcattc agtaaatcca aactgctatg caaaagttat 5701 gatggttaat ggtgaccaca ggataggcat ctttgctaag agggctatcc agactggtga 5761 agagttgttt tttgattaca gatacagcca ggctgatgcc ctgaagtatg tgggcatcga 5821 acgagaaatg gaaatccctt gatctagata attctaccgg gtaggggagg cgcttttccc 5881 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg 5941 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg 6001 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca 6061 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat 6121 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct 6181 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag 6241 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac 6301 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac 6361 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 6421 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 6481 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 6541 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 6601 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 6661 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 6721 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 6781 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 6841 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 6901 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 6961 cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga 7021 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 7081 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 7141 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 7201 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 7261 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 7321 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 7381 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg 7441 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 7501 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc 7561 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga 7621 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa 7681 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac 7741 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt 7801 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc 7861 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg 7921 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac 7981 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta 8041 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag 8101 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca 8161 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca 8221 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 8281 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 8341 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 8401 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 8461 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga 8521 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 8581 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 8641 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 8701 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 8761 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 8821 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 8881 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 8941 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 9001 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 9061 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 9121 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 9181 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 9241 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 9301 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 9361 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 9421 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 9481 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 9541 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 9601 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 9661 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 9721 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 9781 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 9841 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 9901 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 9961 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 10021 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 10081 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 10141 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 10201 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 10261 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 10321 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 10381 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 10441 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 10501 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 10561 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 10621 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 10681 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct 10741 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 10801 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac 10861 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact 10921 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt 10981 taaagaaatt gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta 11041 tttgttaaat atgtactaca aacttagtag t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.