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文献和实验Production of Lentiviral Vector Supernatants and Transduction of Cellular Targets
particles. At top are the three plasmids pHIVPV, pHIV-TV, and the VSV G expression plasmid. A tri-transfection of 293T cells is performed by the calcium-phosphate technique. After 2–3 d, the supernatant is harvested (pseudotyped particles shown), and used
Designing Expression Plasmid Vectors in E. coli
The production of proteins is one of the main applications of genetic engineering in biotechnology. Even though standard cloning procedures are now routine and a large variety of host-vector systems for gene expression are available
Preparation and Quantification of Pseudotyped Retroviral Vector
and biochemical inactivation. A cell line producing VSV-G pseudotyped MuLV vector can be established by transfecting 293T cells expressing Gag, Pol, and VSV-G (293 GPG cell line) with a retroviral vector plasmid. Transduction potency of the resulting VSV-G
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