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文献和实验of xenobiotic carboxylic acids are metabolized to their amino acid conjugates via a pathway that exists primarily in liver and kidney. This conjugation occurs in a two?step pathway catalyzed by two distince types of enzymes, ligases and transferases
Amino-allyl Reverse Transcription
with the amino-allyl dye coupling procedure all Tris must be removed from the reaction to prevent the monofunctional NHS-ester Cye dyes from coupling to free amine groups in solution. Fill one Microcon 30 concentrator with 450 ul water. Add neutralized
Amino-allyl Reverse Transcription
unicorporated/quenched cye dyes proceed with Qia-quick PCR purification kit. Combine Cy3 and Cy5 reactions. Add 70ul water. Add 500ul Buffer PB. Apply to Qia-quick column and spin at 13,000 rpm in for 30-60 sec. Aspirate off flo-thru.
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