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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
BRD7-IN-1 free base
- 库存:
999
- 供应商:
上海麦克林生化科技股份有限公司
- CAS号:
2305379-66-0
- 规格:
10mg
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文献和实验细菌RNA的提取--ISOLATION OF RNA FROM BACTEROIDS
at 8000 rpm. To the supernatant add 0.7 vol isopropanol, chill, spin again, and resuspend pellet in 5 ml.5X Running buffer for RNA (formaldehyde) gels: (For 500 ml) 2 ml 0.5 M EDTA, 6.8 g Na acetate, 12.8 g MOPS (free acid), 9.0 g MOPS (base).Induce cells
mM. pH at g/50ml 1M or g/liter for 0.05 M 5o C 25o C 37o C Tris HCl Tris Base H2 O Tris HCl Tris Base H2 O 7.55 7.00 6.70 7.28 0.47 44.28 7.66 7.10 6.80 7.13 0.57 44.33 7.76 7.20 6.91 7.02 0.67
ISOLATION OF RNA FROM BACTEROIDS
. at -80 C. ISOLATION OF RNA FROM FREE-LIVING RHIZOBIA Cells from 100 ml culture in early log phase GYPC were spun and resuspended in 1 ml sterile 25 sucrose. This solution was transferred to a sterile centrifuge tube and 6.5 ml M-STET, 0.5 ml
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