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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
5,5'-Sulfonylbis(1,3-dibromo-2-(2,3-dibromopropoxy)benzene)
- 库存:
999
- 供应商:
上海麦克林生化科技股份有限公司
- CAS号:
42757-55-1
- 规格:
100mg/250mg/1g/5g
| 规格: | 100mg | 产品价格: | ¥356.0 |
|---|---|---|---|
| 规格: | 250mg | 产品价格: | ¥596.0 |
| 规格: | 1g | 产品价格: | ¥1602.0 |
| 规格: | 5g | 产品价格: | ¥6410.0 |
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文献和实验Single Primer ("Semi-Random") P
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI
X TBE in the gel and 0.6X TBE as the electrophoresis buffer. 2. Run gels at 55 W. Wattage must not exceed 55-60 W even with large 40 x 40 cm plates or a temperature of 50℃. 3. A 10 min prerun is sufficient 4. Maintain gel temperature between 40-50
: 5’TCTGTTGTGTTTCCTCCCTGTTGG3’ Amplify 360 bp of hGH sequence present in 3’ UTR of the transgene. PCR amplification conditions... 27 cycles of 94°C x 60 seconds, 55°C x 60 seconds and 72°C x 90 seconds.
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