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文献和实验Phycoerythrin conjugation protocol
reagents and incubate at 4 °C overnight on rotary mixer. Add 80 µl N-ethyl maleimide (0.1 mM) to reaction mixture to stop reaction. Agitate and incubate 30 mins at room temperature Concentrate to 0.5 ml or less on Centricon
Conjugation of Antibody to HRP
:EDTA Add 0.25 g NaOH pellets to this solution to neutralize to approximately pH 7.0 Add to antibody solution at 6.49 ml/ml SATA. Gently stir for 30 minutes at room temperature. Equilibrate column with PBS:EDTA. Desalt thiolated antibody
water for every mg of transferred ground seed material into the sample tube and vortex to mix. d. Continue to Extract seeds DNA 4) Extract of ground seeds DNA a. Add 45 μl PS1 Buffer and 5 μl PS2 Buffer
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