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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温避光保存
- 保质期:
2年
- 库存:
大量现货
- 供应商:
上海钦诚生物
- CAS号:
107-97-1
- 规格:
20mg
英文别名:Glycine, N-methyl-
分 子 式:C3 H7 N O2
分 子 量:89.09
C A S号: 107-97-1
纯 度:HPLC≥98%
熔 点:212.5 °C (decomp)
沸 点(预测值): 195.1±23.0 °C | Condition: Press: 760 Torr
密度:1.093±0.06 g/cm3 | Condition: Temp: 20 °C Press: 760 Torr
酸度系数:2.36±0.10 | Condition: Most Acidic Temp: 25 °C
储存条件:2-8°C,干燥、避光、密封
规格:20mg 50mg 100mg 500mg 1g 2g等应客户需求包装
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文献和实验Protocols for the preparation of tumour cells for s.c. injections in mice
in ice, providing material for immdiate s.c. injection into 3-4 mice. Cell concentration before injection can be determined using a Neubauer-cytometer (Sigma). Approximately 106 -107 cells are injected subcutaneously into 6-14 week-old female nude mice
).If you assume the efficiency of the cells is between 107 and 108/µg of DNA,then you need to dilute the plasmid to a concentration of 0.1 ng/µl.Then transform 1 ml (0.1 ng)and plate both high (90% of the transformation)and low (10% of the transformation
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of interest. The immunoprecipitated DNA can then be amplified using primers spanning the restriction enzyme site found to be involved in the 3C ligation with other partner(s) in each of the 6C clones. In early attempts of the 6C assay (Tiwari et al. 2008), screening
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