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文献和实验Immunocytochemical Studies of the Retina
It is often desirable to localize a specific protein within a layer, cell, or subcellular organelle in the retina. A variety of immunocytochemical techniques are available to achieve this goal. These techniques all utilize an antibody
Screening of Antigen-Specific Antibody-Secreting Cells
Screening of antigen-specific antibody-producing cells is a key step for obtaining antigen-specific monoclonal antibodies. In murine system, hybridoma between B-lymphocytes and myeloma cells is used to screen and produce antigen-specific
Dissociated Retina Immunohistochemistry
• Add primary antibody (see Dyer and Cepko for dilutions) and incubate for 30 minutes. • Wash 3 times in PBS and add secondary antibody at a dilution of 1:500. • Incubate for 30 minutes, wash 3 times in PBS and add a few drops of ABC reagent
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