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文献和实验We established a sensitive non-radioactive in situ hybridization (ISH) method for the detection of chicken IgG γ-chain mRNA in paraffin sections. RNA probes were transcribed in vitro fromcloned chicken IgG CH1 nucleotide sequences with SP6/T7 RNA
. Directions for preparation of the DNaseI column are below in Appendix . Day 1 Extraction of Actin from Chicken Brain (large scale prep from tissue) 1. Homogenize tissue (fresh or frozen) in 1X Extraction Buffer without detergents at a ratio of 100
Characterizing Somatic Hypermutation and Gene Conversion in the Chicken DT40 Cell System
The secondary immunoglobulin gene diversification processes, somatic hypermutation (SHM), immunoglobulin gene conversion (GCV), and class switch recombination, are important for efficient humoral immune responses. They require the action
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