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文献和实验Optimizing Splice-Switching Oligomer Sequences Using 2-O-Methyl Phosphorothioate Chemistry
and then a set of oligomers complementary to the acceptor and donor splice sites, as well as intra-exonic regions predicted to contain exon splice enhancers, are designed and synthesized as 2′-O-methyl-modified bases on a phosphorothioate backbone (2OMeAOs
Solid-Phase Synthesis of Neuropeptides by Fmoc Strategies
an automated peptide synthesizer that performs solid-phase peptide synthesis using the Fmoc strategy (1 ). This is based on the sequential addition of amino acid residues to an insoluble polymeric support. The base-labile Fmoc group
DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
(dip 3-6 times or leave ~15-60 seconds). The section should not be too dark. The methyl green concentration can be varied if sections are generally too dark or too light. It's better to start with a lower concentration. dH2O 1-5 mins
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