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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海然其生物科技有限公司
- 克隆性:
多克隆
- 抗体英文名:
Anti-GFP antibody
- 抗体名:
Anti-GFP 抗体
- 宿主:
Rabbit
- 适应物种:
Mouse, Rat, Human
- 免疫原:
Recombinant Full Length Protein corresponding to Aequorea victoria GFP.
- 亚型:
IgG
- 形态:
Liquid
- 应用范围:
WB, IHC-FoFr, IHC-P, ELISA, EM, IHC-FrFl, IP, IHC-Fr, ChIP, ICC/IF
- 保存条件:
-20℃~4℃
- 规格:
50ul/500ul
| 规格: | 50ul | 产品价格: | ¥4946.01 |
|---|---|---|---|
| 规格: | 500ul | 产品价格: | ¥46980.88 |
Product SpecificationsAnti-GFP antibody (ab290) is a rabbit polyclonal antibody and is validated for use in ELISA, EM, ICC/IF, IHC-FoFr, IHC-Fr, IHC-FrFl, IHC-P, IP and WB in human samples.
Anti-GFP antibody (ab290) was first used in a scientific publication in 2002 and has been cited over 3370 times in peer reviewed journals. It's performance in Western blot, immunofluorescence and IHC in human and mouse samples is trusted by the scientific community.
GFP antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-GFP antibody (ab290) has been validated in ELISA, Western Blot and imaging applications.
Anti-GFP antibody (ab290) specifically detects GFP (UniProt ID: P42212; Molecular weight: 27kDa) and is sold in a convenient trial size to enable initial testing (50 µL) and larger sizes for subsequent scaling up experiments (500 µL).
Quality and ValidationAbcam's high quality validation processes ensure Anti-GFP antibody (ab290) has high sensitivity and specificity.
Anti-GFP antibody (ab290) has 178 independent reviews from customers.
Target InformationGreen Fluorescent Protein (GFP), originally derived from the jellyfish Aequorea victoria, emits a bright green fluorescence under ultraviolet or blue light. This unique property makes GFP an invaluable tool in molecular and cell biology research. Widely used to tag proteins, GFP allows scientists to visualize and track protein expression, localization, and interactions within living cells. Key applications of GFP include fluorescence microscopy (ICC, IHC, IF), flow cytometry (FC), and western blotting (WB).
形式 Liquid
纯度 Whole antiserum
纯化说明
This antibody is provided as whole antiserum. It is not possible to determine the exact concentration of the anti-GFP antibody ab290, since whole serum contains many other host serum proteins and other antibodies, besides the antibody of interest. The total IgG concentration is 5 mg/mL.
This antibody does not contain an anti-bacterial agent – use sterilized equipment and freeze small aliquots to reduce the risk of contamination.
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 1.25% Sodium chloride
运输条件 Blue Ice
推荐的短期储存时间 1-2 weeks
推荐的短期储存条件 +4°C
推荐的长期储存条件 -20°C
分装信息 Upon delivery aliquot
储存信息 Avoid freeze / thaw cycle
靶点信息Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体?
对照,2道为1:1000倍稀释我们先看一下EarthOx的单克隆GFP抗体——Anti-GFP Tag Monoclonal Antibody [GF28R](货号#E022030),其免疫原是KLH偶联的水母GFP蛋白的N末端多肽,由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致,因此从免疫原上来说,GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是,EarthOx对于该单克隆抗体还进行了明确的验证试验,试验也表明该Anti-GFP Tag Monoclonal
: 这个抗体是非磷酸化的抗体non-phospho-antibody ,33 代表的是基因位点 感觉不应该说“33 代表的是基因位点”,准确的说是指:该抗体的免疫原是合成非磷酸化的β-Catenin的33位丝氨酸附近的多肽。 Anti β-Catenin (Ab-33) Immunogen:Synthesized non-phosphopeptide derived from human β-Catenin
Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
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