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文献和实验in vitro assays of a cell population’s response to external factors. The reduction of tetrazolium salts is now widely accepted as a reliable way to examine cell proliferation. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay
reader).ReferenceMosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983 Dec 16;65(1-2):55-63.
CellTrace CFSE Cell Proliferation Kit
. 实验步骤 For staining cells prior to flow cytometric analysis of cell proliferation or cell division, the protocol below is appropriate. More information on this procedure can be found in reference 7. Our suggested initial conditions
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