Recombinant protein of human c

High-Throughput Assays to Measure Intracellular Ca2+ Mobilization in Cells that Express Recombinant S1P Receptor Subtypes

Intracellular Ca2+ mobilization is a useful readout to screen for agonists or antagonists of G-protein �coupled receptors (GPCRs). Here, we describe methods to conduct high-throughput screening of stably or transiently transfected HTC4 cells

Human Olfactory Receptors: Recombinant Expression in the Baculovirus/Sf9 Insect Cell System, Functional Characterization, and Odorant Identification

9 insect cell system allows this protein to be expressed at the cell surface. We used Ca2+ imaging to demonstrate that recombinant OR 17-210 produces cellular activities upon odorant stimulation with ketones. Furthermore

ANTIBODY BINDING TO PROTEIN A AND PROTEIN G

of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G