- ￥1024 - 1824
- 正能
- 126146
- 中国
- 2026年03月05日
- WB,IHC-P
- Human,Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
WB,IHC-P
- 适应物种：
Human,Mouse
- 标记物：
Unconjugated
- 保存条件：
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- 形态：
Liquid
- 规格：
50μl/100μl
| 规格： | 50μl | 产品价格： | ￥1024.0 |
|---|---|---|---|
| 规格： | 100μl | 产品价格： | ￥1824.0 |
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文献和实验Myosin Light Chain Preparation
1 and 2 with the Coomassie Blue Assay and B.S.A.standards.Run 10% SDS gels. 25.Do 32P assay to determine maximal extent of phosphorylation of light chain. 26.Scan gels with spectrophotometer at A 600.Cut out and weigh the peaks to determine % impurities and percent
Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
Generation of Rabbit Immune Libraries
for the combinatorial cloning of the variable heavy and light chain immunoglobulin repertoire of rabbits for the subsequent phage display of rabbit scFv antibody fragments is described.
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