- 询价
- Eagle Biosciences
- 美国
- 13S00-A100
- 2026年03月05日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4℃
- 保质期：
1-2 years
- 英文名：
13(S)-HODE Polyclonal Antibody
- 库存：
999
- 供应商：
Eagle Biosciences Inc.
- 规格：
96T or More
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文献和实验Polyclonal and Monoclonal Antibodies
Polyclonal Antibody Immunization of Mice: Injections should be made at intervals of at least two weeks.. We have been using either of the two following adjuvants with success: Freund adjuvant and MPL+TDM adjuvant ( RIBI ImmunoChem
12. Add 20 µl of 5mM chloramphenicol in buffer I(or 20 µl of buffer I for a blank). 13. Incubate 5 min @ 37℃ 14. Make the following mix: 0.75 µl of 4mM acetyl CoA 2.0 µl 750 µM HCl (10X) 16.25 µl H2 O
Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Column （BIO-RAD cat. #732-6008）Oligo dT celluloseRNase-free water5.0 M NaCl0.5 M EDTA1.0 M Tris, pH 7.410% SDS70% EtOHBefore starting protocol1. Measure out 75 mg of oligo
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