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- Sunrise Science
- 美国
- 71-023
- 2026年03月09日
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- 英文名：
anti-RagC / RRAGC antibody, rabbit polyclonal, 100 ul(Sunrise Science; CAT# 71-023)
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Sunrise Science
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文献和实验Purification Of Anti Peptide Antibody
a parastaltic pump and recirculate the serum ON or just batch bind the serum ON. Wash with 5 col vols TBS. Wash with 10 col vols 0.5 M NaCl, 20mM TrisCl pH 7.4, 0.2 Triton- X-100. Wash 5 col vols TBS Elute with 0.15 M NaCl, 0.2 M Glycine
Polyclonal and Monoclonal Antibodies
or O.N at 4¡C. 4) SECONDARY ANTIBODY a) Wash unbound antibody 4 times with PBS + 0.1% Tween 20. b) Add 100 ul of enzyme linked antibody to all wells. Do the appropriate dilutions in the Elisa buffer. (ex: HRP is 2000X). c
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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