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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
iST RFP-Trap Kit for AP-MS sample preparation of RFP-fusion proteins
- 保存条件：
NULL
- 规格：
8rxns
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文献和实验Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
in preparation). This explains why the GBP does not recognise unfolded or denatured GFP (e.g. on immunoblots). For immunoprecipitations of GFP fusion proteins we coupled the GBP covalently to monovalent matrices (e.g. agarose beads or magnetic particles
pipette if necessary. Optional: rinse out the wells with running buffer using a P200 pipetteman. 8. Sample preparation. Mix protein with 1 volume or more of sample buffer, and boil in a boiling water bath for 2-5 min. Can freeze the sample at this point
Studies of the Ubiquitin Proteasome System
of Wheat E1 in E. coli PET3a, pET15b or other vector for expression of proteins in E. coli from a T7 promoter but without a protein sequence Basic Protocol 21: Preparation of Mouse E1 in Insect Cells Using a Baculovirus
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