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- Adipogen
- 瑞士
- SBB-PS0009-M002
- 2026年03月03日
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- 详细信息
- 文献和实验
- 技术资料
- CAS号：
/
- 英文名：
Ac-Ala-Asn-Trp-AMC [Ac-ANW-AMC]
- 保质期：
Stable for at least 1 year after receipt when stored at -20°C.
- 保存条件：
-20°C
- 库存：
少量现货
- 供应商：
欣博盛生物
- 规格：
2 mg
Ac-Ala-Asn-Trp-AMC [Ac-ANW-AMC]产品由Adipogen公司生产，产地是瑞士。该产品货号为SBB-PS0009-M002，包装规格是2 mg。如需购买该产品，询问折扣价/货期/技术问题，请联系Adipogen授权代理商——欣博盛生物
Ac-ANW-AMC is a fluorogenic peptide substrate for measuring chymotrypsin-like activity of the immunoproteasome. Hydrolysis of this substrate by the β5i-subunit of the immunoproteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm. This substrate is specific to the immunoproteasome and is not hydrolyzed efficiently by the constitutive proteasome.
Synonyms Proteasome Substrate
Product Type Chemical
Properties
Formula
C30H32N6O7
MW 588.6
Sequence
Acetyl-Ala-Asn-Trp-7-amido-4-methylcoumarin
Purity Chemicals ≥95% (HPLC)
Appearance Lyophilized powder.
Solubility Soluble in DMSO.
Declaration Manufactured by South Bay Bio.
Other Product Data
Use: After preparing a stock solution in DMSO (≥10mM) store product at -20°C to -80°C. It is recommended to make multiple aliquots after the first thaw to ensure best performance. Chymotrypsin-like activity can be measured using a typical working concentration range from 10-50µM.
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key ORTKHFLJSJQDKO-ZCWWJEROSA-N
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Protect from light.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
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文献和实验- Elucidating the catalytic subunit composition of distinct proteasome subtypes: A crosslinking approach employing bifunctional activity-based probes: C.K. Cornish, et al.; ChemBioChem 16, 284 (2015)
- Pharmacodynamic monitoring of (immuno) proteasome inhibition during bortezomib treatment of a critically ill patient with lupus nephritis and myocarditis: K.A. De Groot, et al.; Lupus Sci. Med. 2, e000121 (2015)
- Immunoproteasome β5i-selective dipeptidomimetic inhibitors: P.K. Singh, et al.; ChemMedChem 11, 2127 (2016)
- Acetaldehyde suppresses the display of HBV-MHC class I complexes on HBV-expressing hepatocytes: M. Ganesan, et al.; Am. J. Physiol. Gastrointest. Liver Physiol. 317, G127 (2019)
- Monitoring the immunoproteasome in live cells using an activity-based peptide–peptoid hybrid probe: B.L. Zerfas & D.J. Trader; J. Am. Chem. Soc. 141, 5252 (2019)
， 5~10min/次?→ECL显影或NBT/BCIP显色。结果如图9-2： 2 荧光分光光度计分析 原理：活化的Caspase-3能够特异切割D1E2V3D4-X底物，水解D4-X肽键。根据这一特点，设计出荧光物质偶联的短肽Ac-DEVD-AMC。在共价偶联时，AMC不能被激发荧光，短肽被水解后释放出AMC，自由的AMC才能被激发发射荧光。根据释放的AMC荧光强度的大小，可以测定caspase-3的活性，从而反映Caspase-3被活化的程度。方法：收获细胞正常
， 5~10min/次？→ECL显影或NBT/BCIP显色。结果：2、荧光分光光度计分析原理：活化的Caspase-3能够特异切割D1E2V3D4-X底物，水解D4-X肽键。根据这一特点，设计出荧光物质偶联的短肽Ac-DEVD-AMC.在共价偶联时，AMC不能被激发荧光，短肽被水解后释放出AMC，自由的AMC才能被激发发射荧光。根据释放的AMC荧光强度的大小，可以测定caspase-3的活性，从而反映Caspase-3被活化的程度。 方法：收获细胞正常或凋亡细胞，PBS洗涤，制备细胞裂解液，加Ac
[hCys-（N-Me）Phe -Tyr-D-Trp-Lys-Val]的合成也是应用HBTU为缩合剂，反应路线如下： 2.3 Bop法 McMurray等以Cyclo（Asp-Asn-Glu-Tyr-Ala-Ala-Arg-Gln-D-Phe-Pro）（Tyr I+1）为研究对象，以便确定Tyr I+1的哪些位置对蛋白酪氨酸激酶的亲和性是必不可少的，以及哪些氨基酸对活性贡献最大，以Bop/HOBt为复合缩合剂，在1mmol/L浓度下，合成了一系列Tyr I+1类似物，并经磷酸化和亲和力实验，发现6位
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