Protein G 磁珠 | NEB

品牌	货号	产品中文名称	产品英文名称
NEB	S1430	Protein G 磁珠	Protein G Magnetic Beads

货号	产品中文名称	产品英文名称	规格
S1430S	Protein G 磁珠	Protein G Magnetic Beads	1 ml

产品特点

Protein G Magnetic Beads are an affinity matrix for the small-scale isolation and purification of immunoglobulins. Specifically, the beads consist of a truncated form of recombinant Protein G that is covalently coupled to a nonporous superparamagnetic particle.

• Exhibits high affinity for subclasses of IgG from many species including human, rabbit, mouse, rat and sheep
• Can be used for immunoprecipitations with mouse monoclonal antibodies
• The protein is coupled through a linkage that is stable and leak resistant over a wide pH range. This permits the immunomagnetic purification of IgGs from ascites, serum or cell culture supernatants
• The matrix can be regenerated without loss of binding capacity
• Can be used to immunoprecipitate target proteins from crude cell lysates using a selected primary antibody. In addition, specific antibodies can be chemically cross-linked to the Protein G coated surface to create a reusable immunoprecipitation bead, thereby avoiding the co-elution of antibody with the target antigen
• Binding capacity: 1 ml of Protein G Magnetic Beads binds >280 µg of Human IgG
• Quick, small-scale purification of many subclasses of IgGs affords higher throughput and efficiency

Protein G 磁珠是一种用于少量分离和纯化免疫球蛋白的亲和基质。截短型的重组 Protein G 可与无孔超顺磁微粒共价偶联。Protein G 对许多物种的 IgG 亚类表现出高亲和性，包括人、兔、小鼠、大鼠和羊（1）。此外，Protein G 可用于小鼠单克隆抗体的免疫沉淀反应（1）。由于 Protein A 与磁珠的偶联在较宽的 pH 范围内都是稳定且致密的，因此可以对来自腹水、血清或细胞培养上清物中的 IgG 进行磁性免疫纯化，基质随后还可以进行再生而不影响结合容量。Protein G 磁珠还可以与选定的一抗联合使用从细胞粗提裂解物中免疫共沉淀目的蛋白。另外，特异性抗体可以化学交联到 Protein G 包被的表面上，形成可重复使用的免疫沉淀磁珠，从而避免抗体与靶标抗原一起被洗脱（2、3）。

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产品类别:

富集,

染色质分析,

Magnetic Beads and Racks,

DNA 甲基化分析

应用:

Pull Down Assays,

High-throughput cloning and automation solutions

备注：以上仅为 S1430 的部分产品信息，如需NEB货号 S1430 的完整产品信息及相关的实验操作手册等等，请通过NEB官网查找对应的货号获取。