NEBExpress® 镍离心柱 | NEB

品牌	货号	产品中文名称	产品英文名称
NEB	S1427	NEBExpress® 镍离心柱	NEBExpress®Ni Spin Columns

货号	产品中文名称	产品英文名称	规格
S1427L	NEBExpress® 镍离心柱	NEBExpress Ni Spin Columns	25 each
S1427S	NEBExpress® 镍离心柱	NEBExpress Ni Spin Columns	10 each

产品特点

• Includes ready-to-use pre-packed Ni spin columns and all required buffers for purification
• Purification of ≥1 mg His-tagged protein per column in as little as 15 minutes
• High specific binding of His-tagged proteins yielding purities of >95%
• Strong nickel ion binding provides excellent resistance to EDTA and reducing agents. Compatible with commercially available detergent-based cell lysis reagents
• Isolation and purification of His-tagged fusion proteins under native or denaturing conditions

Spin columns containing an affinity matrix for the small-scale isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. Immobilized Metal Affinity Chromatography (IMAC) purifications employing NEBExpress® Ni Spin columns can be performed under native or denaturing conditions, yielding highly pure target in a single protein purification step.  This enables screening of expression conditions and streamlines the functional and structural characterization of the target protein.

Support Matrix: Spherical, agarose based microparticles ranging in size from 10-100 μm.

备注：以上仅为 S1427 的部分产品信息，如需NEB货号 S1427 的完整产品信息及相关的实验操作手册等等，请通过NEB官网查找对应的货号获取。