酪氨酸激酶 II（CK2） | NEB

品牌	货号	产品中文名称	产品英文名称
NEB	P6010	酪氨酸激酶 II（CK2）	Casein Kinase II (CK2)

货号	产品中文名称	产品英文名称	规格
P6010L	酪氨酸激酶 II（CK2）	Casein Kinase II (CK2)	200,000 units
P6010S	酪氨酸激酶 II（CK2）	Casein Kinase II (CK2)	40,000 units

产品特点

Casein Kinase II (CKII) is a constitutively active serine/threonine protein kinase composed of two 44 kDa catalytic α-subunits and two 26 kDa regulatory β-subunits in an α2β2 configuration to form stable heterotetramers. The general recognition motif for phsophorylation by CKII is SXXE/D.

• Recombinant enzyme with no detectable protease or phosphatase contaminating activities
• Optimal activity and stability for up to 12 months

Casein Kinase II (CK2) is a constitutively active serine/threonine protein kinase composed of two 44 kDa catalytic α-subunits and two 26 kDa regulatory β-subunits in an α₂β₂ configuration to form stable heterotetramers. CK2 holoenzyme undergoes autophosphorylation at two serine residues (S2/S3) of its β-subunit. Recently it has been shown that CK2 α-subunits undergo intermolecular tyrosine-autophosphorylation at Y182, which may represent a specific regulatory mechanism. Also, CK2 is able to phosphorylate, under special circumstances, tyrosyl residues in proteins. CK2 is implicated in a variety of cellular functions (1,2).

产品来源

Isolated from a strain of E. coli expressing both α and β CK2 subunits derived from a human glioblastoma cDNA library (kindly provided by Dr. D. Marshak) (3).

识别决定因素

The CK2 substrate specificity is invariably determined by multiple acidic residues located at positions between -2 and +5 relative to the target amino acid (mostly Ser and rarely Thr). The general recognition motif for phsophorylation by CK2 is SXXE/D, although SXE/D and S/D, and variations of these sequences are also phosphorylated. Polyanionic compounds, like heparin, inhibit CK2 activity with a K_i of 1.4 nm (4,5).

产品类别:

Protein Kinases

应用:

Protein Phosphatases and Kinases

备注：以上仅为 P6010 的部分产品信息，如需NEB货号 P6010 的完整产品信息及相关的实验操作手册等等，请通过NEB官网查找对应的货号获取。