- 询价
- Agrisera
- Sweden
- AS21 4635
- 2026年03月02日
- Dot blot (Dot), ELISA (ELISA),Immunocytochemistry (IHC), (ICC),Immunohistochemistry (paraffin), Western blot (WB)
- Rabbit
- Escherichia coli
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Ribonucleic acid polymerase isolated and purified from Escherichia coli, UniProt: P0A7Z4
- 形态：
Biotin-conjugated, purified IgG in PBS, pH 7.2.
- 保存条件：
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sid
- 克隆性：
PolyclonalRibonucleic acid polymerase isolated and purified from Escherichia coli, Freund's complete adjuvant is used in the first step of the immunization procedure
- 适应物种：
Escherichia coli
- 供应商：
Agrisera
- 宿主：
Rabbit
- 应用范围：
Dot blot (Dot), ELISA (ELISA),Immunocytochemistry (IHC), (ICC),Immunohistochemistry (paraffin), Western blot (WB)
- 靶点：
Anti-Ribonucleic acid polymerase, biotin conjugated, Escherichia coli
- 抗体英文名：
Anti-Ribonucleic acid polymerase, biotin conjugated, Escherichia coli
- 抗体名：
Anti-Ribonucleic acid polymerase, biotin conjugated, Escherichia coli
- 规格：
10 mg
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文献和实验A bench-scale protocol for the purification of plasmid DNA (pDNA) produced in Escherichia coli is described. The method is specifically designed to prepare pDNA vectors for gene therapy and DNA vaccination applications. The method comprises
Preparation of nucleic acid probes
and purified. Larger DNA fragments can be end-labeled by various alternative methods. Fill-in end-labeling (Figure 5.3B ) is one popular approach, and uses the Klenow subunit of E. coli DNA polymerase. Again, fragments carrying label at one end
Strategies to Optimize Protein Expression in E. coli
, R.B., and Waugh, D.S. 2001. Single amino acid substitutions on the surface of Escherichia coli maltose‐binding protein can have a profound impact on the solubility of fusion proteins. Protein Sci. 10:622‐630.
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