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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
- 形态:
Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.
- 保存条件:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
- 克隆性:
Polyclonal
- 适应物种:
Staphylococcus aureus
- 供应商:
Agrisera
- 宿主:
Rabbit
- 靶点:
Anti-Cas9 | CRISPR-associated endonuclease 9 (polyclonal) - Biotin conjugated 40 µg
- 抗体英文名:
Anti-Cas9 | CRISPR-associated endonuclease 9 (polyclonal) - Biotin conjugated 40 µg
- 抗体名:
Anti-Cas9 | CRISPR-associated endonuclease 9 (polyclonal) - Biotin conjugated 40 µg
- 规格:
40 µg
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
Dual- and Triple-Color Fluorospot
for the other cytokine (Fig. 1). As a second step in the detection, streptavidin conjugated to a red fluorophore (Cy3) and anti-FITC antibodies labeled with a green fluorophore, respectively, are used (10, 11). The resulting spots are subsequently analyzed using
Immunofluorescent Staining of Mouse and Rat Leukocytes
(or 50 µl wash buffer for negative controls). Mix by gently vortexing or tapping. Incubate at 4°C for 20-40 min in the dark. Wash 2X with 200 µl wash buffer (or 3X if a biotin-conjugated primary antibody is used). After each centrifugation, 350 x g
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