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- NEB
- N3019
- 2026年04月02日
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| 品牌 | 货号 | 产品中文名称 | 产品英文名称 |
|---|---|---|---|
| NEB | N3019 | Lambda DNA-Mono Cut Mix | λ DNA-Mono Cut Mix |
| 货号 | 产品中文名称 | 产品英文名称 | 规格 |
|---|---|---|---|
| N3019S | Lambda DNA-Mono Cut Mix | Lambda DNA-Mono Cut Mix | 100 gel lanes |
产品特点
- The Mono Cut Mix is a mixture of intact lambda DNA and lambda DNA separately digested with ApaI, KpnI, XbaI and XhoI.
- The fragments have been filled in with DNA Polymerase I Large (Klenow) Fragment to prevent re-annealing.
- The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
- For pulsed-field gel electrophoresis (PFGE)
- May also be used for standard electrophoresis
- Size range: 1,503 bp to 48,502 bp
- Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
- 建议的凝胶和电泳迁移条件：在 CHEF 系统上进行 PFGE，1% 0.5X TBE 琼脂糖，6 V/cm，15℃，0.5 到 1.5 秒，持续 20 小时。
备注：以上仅为 N3019 的部分产品信息，如需NEB货号 N3019 的完整产品信息及相关的实验操作手册等等，请通过NEB官网查找对应的货号获取。
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文献和实验Purification of DNA from lambda phage
Sodium Acetate pH 4.8 and 2 vol of room temperature 100% ethanol. Mix,microcentrifuge 10 min to precipitate DNA . G.Add 1 ml 70% ethanol and centrifuge 5 min.Dry in speed vac about 5 min,dissolve in 100 pl TE.Yield of DNA should be around 2OItg per 17
David Harry Institute of Forest Genetics USDA Forest Service Pacific Southwest Research Station August 26, 1993 Background : There are many published methods for mini-preps of DNA from lambda phage cloning vectors. This one has worked
cDNA AMPLIFICATION FROM LAMBDA-PHAGE LIBRARY
PREPARE SOLUTIONS 1. SM buffer (1 L): Mix 5.8 g of NaCl, 2 g of MgSO4 -7H2 O, 50 mL of 1M Tris-HCl, pH 7.5, 0.5 mL of 2% gelatin, and dH2 O to 1 L (Autoclave) 2. TENS
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