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文献和实验should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
Figure 2 In vivo silencing of murine apoB mRNA by siRNAs in wild-type mice. Full legend High resolution image and legend (104k) Silencing of the apoB mRNA would be expected to result in a corresponding reduction in apoB protein levels. An ELISA
protein levels should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice
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