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999
- 供应商:
Alpha Diagnoestic International
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- 规格:
100 ul
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文献和实验Isolation of Microtubules (Bovine Brain)
LEVEL II Materials Freshly removed bovine brain 2 Wire sieve (tea strainer) Microtubule buffer (MT buffer) 0.1 M MES (2-(N-Morphilino)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis
Immunofluorescent Localization of Tubulin
anti-tubulin dilution using PBS. PbS alone may be used or better, augment the PBS with 3% (w/v) Bovine Serum Albumin (BSA). It may be necessary to check the appropriate antibody dilution. If so, make 1/10, 1/100, 1/1,000 and 1/10,000 dilutions
Immunofluorescent Localization of Tubulin
and rinse 2X with PBS. Prepare a 1/40 anti-tubulin dilution using PBS. PbS alone may be used or better, augment the PBS with 3% (w/v) Bovine Serum Albumin (BSA). It may be necessary to check the appropriate antibody dilution
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