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文献和实验Preparation of Recombinant HIV-1 Gag Protein and Assembly of Virus-Like Particles In Vitro
The mechanism of assembly of retroviruses is not fully understood. Purification of retroviral Gag protein and studying its solution state and assembly properties might provide insights into retroviral assembly mechanisms
Quantitation of HIV-1gag DNA and RNA From Single Frozen Cell Pellets
(4 , 5 ), disease progression (6 –8 ), and propensity for the transmission of disease (9 ,10 ) as well. Now accepted as a surrogate for all of these features of HIV-1 disease, specific guidelines have been adopted for the use of viral load measures
Effective Pol III-Expressed Long Hairpin RNAs Targeted to Multiple Unique Sites of HIV-1
mutable viruses such as HIV-1. A combinatorial approach is therefore required for long-term inhibition of gene expression. RNA Pol III-driven long hairpin RNA (lhRNA) duplexes can be cleaved several times by Dicer, yielding multiple functional siRNAs
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