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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
500 mL
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文献和实验Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr(图)
This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
Cellular lysis solution ChIP diluent Cloning vector The vector used should have enzyme overhangs similar to those generated in the 3C assay. Such vectors can be custom-constructed or obtained commercially. For example, for cloning
in vitro assays of a cell population’s response to external factors. The reduction of tetrazolium salts is now widely accepted as a reliable way to examine cell proliferation. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay
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