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- 2026年02月23日
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- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
1
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文献和实验Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
from lab stock) PC12 Transfection Media DMEM (Gibco/BRL # 11960-028; use fairly new bottle so pH is consistent) 10% calf serum 2 mM glutamine (1/100 from lab stock) penn/strep (1/200 from lab stock) make fresh, day of transfection Plasmid DNAs
) protein, load only 0.0125 OD ml. For Drosophila tissue culture cells, suspend 3X106 cells per 90 µl cracking buffer, and load about 20 µl per lane (0.8 mm thick gel with the BRL gel box and 20 well comb). 10. Running. Use a constant Amps power supply
in gel tray about 1 inch from one end of the tray and position the comb vertically such that the teeth are about 1-2 mm above the surface of the tray Pour 50EC gel solution into tray to a depth of about 5 mm. Allow gel to solidify about 20 minutes
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