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- Gelcompany
- A1-G-24-100
- 2026年02月23日
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- 文献和实验
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10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
1
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文献和实验(optional; see Steps 21 and 37) Horizontal gel electrophoresis tank for agarose gels (for duplex PCR only; see Steps 40-53) Standard DNA sequencing gel apparatus with 31 x 38.5-cm glass plates, 0.4-mm spacers, and a shark's-tooth comb (for hot-stop PCR
The ribonuclease protection assay (RPA)
., 5 mm well width). Use of a sharks tooth comb is not recommended. 23. After polymerization (~1 hour), remove the comb and flush the wells thoroughly with 0.5X TBE. Place each gel in a vertical rig (use a gel set up that has a heat dispenser
PREPARATION OF SEQUENCING GELS
MATERIALS: 2-glass plates 1 sharks -tooth comb and spacers Whatman 3 mm paper 30 or 40% acrylamide-bis (19:1) 10X TBE urea 10% ammonium persulfate TEMED 60 cc. syringe Rain-ex Preparation of glass plates: Wash and rinse
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