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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
60
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文献和实验SDS-PAGE Western Blotting Protocols
glass plate can be treated with Rain-X to facilitate later removal of the gel. 2. Slide into holder without tightening the screws. 3. Place on pouring stand, making sure that the glass plates are pushed all the way to the bottom before tightening
的dNTP则保留在层析柱中。取0.5ul己纯化的探针点样于DE8-paper. 5.8.8 测比活性(试剂比活要求:106 cpm/ml)。 5.9 预杂交: 5.9.1 将预杂交液在杂交炉中68℃预热,并漩涡使未溶解的物质溶解。 5.9.2 加入适当的ULRAhyb到杂交管中(以100 cm2膜面积加入10 ml ULRAhyb杂交液),42℃预杂交4 hr。 5.10 探针变性: 5.10.1 用10 mM EDTA将探针
Western Blotting and Immunostaining
away trapped air between the gel and membrane. If necessary, reposition the membrane once. Working quickly, place a soaked piece of blotting paper on top of the membrane, followed by 3 to 4 soaked blotting pads. The last pad should be about 1 cm
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