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- AffinityImmuno
- ab-131-380
- 2026年02月23日
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- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
500 µg
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文献和实验secondary antibody review -- data from 99 publications
Signaling 18 IgG Alexa Fluor 488 immunocytochemistry 1:2000 18 IgG Alexa Fluor 568 immunocytochemistry 1:2000 19 donkey IgG HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
, and apply primary Ab only to the coverslip. Incubate 45'. Dilute antibody with Blotto. Some typical dilutions are listed below: monoclonal M1 mouse anti-Flag antibody (Sigma , Covance ) (Ca+2 sensitive) 1:350-1:500; polyclonal 12CA5 mouse anti-HA (Roche
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