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文献和实验Protein Carbonyl Measurement by ELISA
) (2,3). Protein-bound DNP can also be measured with increased sensitivity by enzyme-linked immunosorbent assay (ELISA) (4,5). Samples containing protein are reacted with DNP, then the protein is nonspecifically adsorbed to an ELISA plate. Unconjugated DNP
imaging. References that discuss the vagaries of acquiring and analyzing FRET between individually tagged molecules are provided. Curr. Protoc. Protein Sci. 56:19.5.1?19.5.12. © 2009 by John Wiley & Sons, Inc. Keywords: FRET
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
extracellular protein interactions are critical for cellular recognition processes, but are not generally detected by methods that can be applied in a high?throughput manner. This unit describes a protein microarray platform that significantly improves
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