5-(Aminomethyl)-2-(2,6-dioxopi

Cell Proliferation Assay

  Introduction Living (metabolically active) cells reduce tetrazolium salts to colored formazan compounds; dead cells do not. Thus, tetrazolium salt-based colorimetric assays detect viable cells exclusively. Because they are sensitive

【转贴】 从纯化到星辰---冷泉港(CSHL)蛋白质纯化课侧记（全文完）后附全文doc文档

站: Unknown Space - 未名空间 (Fri Apr 29 11:14:36 2005) WWW-POST 本文献给YL （八）”液体DEAE“ 　绕了这么大一圈，现在再回到第一个模块的实验来。前面提到Dr.Burgess要我们 试Gudn HCl溶解sigma32之后的重折叠，结果很糟糕。我们接着试了用sarkosyl做 变性剂的蛋白重折叠。步骤和前一个类似，只是这一次是突然稀释至25倍体积。效果 好了很多，至少没有浑浊现象了。接着我们用Poros HS 50，一种

Oligodeoxynucleotide Containing S‐Functionalized 2′‐Deoxy‐6‐Thioguanosine: Facile Tools for Base‐Selective and Site‐Specific Internal Modification of

‐Deoxyguanosine Basic Protocol 2: Preparation of 3‐Chloromethylene‐4‐Phenylbutane‐2,4‐Dione Basic Protocol 3: Preparation of 2′‐Deoxy‐6‐Thioguanosine Containing Oligodeoxynucleotides and Its Functionalization