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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
北京柏瑞图科技有限公司
- 规格:
ARS-AS21 4683-50ug
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文献和实验Identification of Thioredoxin Target Disulfides Using Isotope-Coded Affinity Tags
quantification of Trx target disulfides in complex protein extracts is described. The procedure utilizes the isotope-coded affinity tag (ICAT) reagents containing a thiol reactive iodoacetamide group and a biotin affinity tag to target peptides containing reduced
Protein Chip for Detection of DNA Mutations
for detection of single-base mismatches and unpaired bases of DNA was developed using a genetic fusion molecular system Trx-His6 -(Ser-Gly)6 -Strep tagII-(Ser-Gly)6 -MutS (THLSLM). The THLSLM coding sequence was constructed by attaching Strep tag II and mutS
有关Production of soluble mammalian proteins in Escherichia coli的经验的一篇好文章
level soluble expression. Thioredoxin (Trx) and maltose binding protein (MBP) were the best N-terminal protein fusions to promote soluble expression, but MBP was most effective as a C-terminal fusion.63 of 95 mammalian proteins expressed at soluble
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