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- Agrisera
- ARS-AS20 4441-HRP
- 2026年02月22日
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- 文献和实验
- 技术资料
- 供应商：
北京柏瑞图科技有限公司
- 规格：
ARS-AS20 4441-HRP-40ug
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
To immunostain using enzyme-coupled secondary antibodies: i. Rinse slides in PBS and remove excess solution. ii. Add 40 µL of secondary antibody (e.g., anti-rabbit IgG [Fc] HRP conjugate, 1:100 dilution
exposure times. The HRP conjugated secondary followed by DAB detection method offers no advantages; it's not very sensitive. Chemiluminescence can be substantially more sensitive than alk. phos. There are a couple of chemistries available
with 300 µL or 100 m L of the appropriate anti-DIG antibody solution for 2 h. (see Notes 3 ). If an HRP-conjugated antibody is used in Step 2 , rinse embryos once with PBTB following the antibody incubation, then perform a nuclear counter stain
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