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文献和实验Anode and cathode buffers (see reciperecipes ) First dimension BN‐gel containing the protein bands of sample Separating and sample gel solutions (Table 19.18
Strategies to Optimize Protein Expression in E. coli
; expression strains and vectors; folded protein; active protein GO TO THE FULL PROTOCOL: PDF or HTML at Wiley Online Library
. Colloidal iron stains are unpredictable. Alcian blue stains are simple, but have a lot of background staining. Stains for biogenic amines Cells that produce polypeptide hormones, active amines, or amine precursors (epinephrine, norepinephrine
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