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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4-25℃
- 保质期:
1 Year
- 英文名:
Master Mix for Spiked Reactions
- 规格:
10mL
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文献和实验amounts of each component per reaction. Make enough master mix for (n+1) reactions. 2.5 l 10X amplification buffer 2.5 l 2mM dNTPs 1.5 l 25mM MgCl2 0.12 l 5U/ l Taq polymerase (0.6 Units/reaction) 1.25 l primer
Single tube confirmation PCR protocol
are for isolate #1, 6-10 are for isolate #2, ..., and tubes 16-20 are for the wild-type control. Each of the 20 tubes should contain 10 µl of the primer mix (5 µl of each primer). - Add the 5 µl of the Zymo treated cells to each of the 20 PCR reactions
(BD Biosciences / Clontech). SYBR Green PCR master mix, 200 reactions (Applied Biosystems). Optical tube and cap strips (Applied Biosystems). SuperScript
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