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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4-20℃
- 保质期:
6个月
- 英文名:
Violet Red Bile Agar Plate
- 供应商:
江苏天净沙
- 规格:
10个9cm
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文献和实验the polylysine coating of the plate. On this noble agar coated, anchorage independent tissue culture plates normal and cancer cells will be seeded in very low density to prevent the contact between cells. Plating efficiency is a measure of the clonogenic ability
SOFT AGAR ASSAY FOR COLONY FORMATION
3. You require 5,000 cells/plate, therefore you need 20,000/tube. Adjust cell count to 200,000 cells /ml. 4. Add 0.1ml of cell suspension to 10ml yellow capped centrifuge tubes. 5. Label 35mm petri dishes with base agar appropriately (it is a good idea
Figure 2). Red/ET competent bacteria carrying the mLhcgr -BAC were transformed with the I-SceI-CS PCR product and plated on LB agar plates supplemented with Km/Cm. Positive clones were screened by PCR (primers mLhcgrP-F/mLhcgr3-R) and further selected
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