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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2年
- 保质期:
1-3天
- 英文名:
常温
- 供应商:
江苏天净沙
- 规格:
250mL
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文献和实验b-Gal Staining of Murine Embryos
PO4.H2O, 77ml of 0.2M Na2HPO4 and add 100ml DDW. the pH will be approx 7.3 0.5M EGTA in 0.1M Phosphate buffer, pH increase to 7. FW 380.4, so 19.02g for making 100ml of 0.5M EGTA. Ethylene Glycol-bis (b-aminoethyl ether)N,N,N',N'-tetraacetic acid
to 7.0 Distilled water 100 ml Store at room temperature 0.5 M EGTA Stock* 19.02 g EGTA (Sodium Salt) distilled water to 90 ml Adjust pH to 7.0 Distilled water to 100 ml Store at room temperature 1M dithiothreitol (DTT)* 1.542 g
EGTA (make 1M and dilute with 4M NaOH till dissolves. Check pH=neutral, make up volume for 0.5M) 0.5M K3Fe (CN)6 = 1.65 g/10 ml (red) 0.5M K4Fe(CN)6 = 2.10 g/10ml (yellow) 40 mg/ml X-gal (in dimethylformamide) 1M MgC12
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