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- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
1 x 96 wells
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文献和实验capture antigens. Based on chemically modified pepti des, SmartBlock™ - in contrast to blocking reagents mainly containing larger molecules - is able to form a blocking layer that also prevents smaller assay components from attaching to free binding
of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM
Spectroscopic Methods for the Determination of Protein Interactions
., and Hu, H.Y. 2003. Study of protein‐protein interactions by fluorescence of tryptophan analogs: Application to immunoglobulin G binding domain of streptococcal protein G. Biopolymers 72:116‐122.
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