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- 库存:
10
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北京柏瑞图科技有限公司
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10 mg
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文献和实验A bench-scale protocol for the purification of plasmid DNA (pDNA) produced in Escherichia coli is described. The method is specifically designed to prepare pDNA vectors for gene therapy and DNA vaccination applications. The method comprises
Strategies to Optimize Protein Expression in E. coli
, R.B., and Waugh, D.S. 2001. Single amino acid substitutions on the surface of Escherichia coli maltose‐binding protein can have a profound impact on the solubility of fusion proteins. Protein Sci. 10:622‐630.
Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) for Cellular Retinoid-Binding Proteins
properties of these proteins are well-known, largely as a result of biochemical studies on protein purified from tissues or expressed in Escherichia coli (see Chapters 8 –10 ), their functions and interactions with other cellular proteins are still uncertain
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