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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
200 ug
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文献和实验Large Scale Tubulin Preparation
itself. I. Tubulin Prep Outline II. Buffers & Nucleotides III. Pouring a 1L Phosphocellulose (PC) Column IV. Brains V. Centrifuges & Rotors VI. Protocol
Large Scale Tubulin Preparation
form before details about the prep itself. I. Tubulin Prep Outline II. Buffers & Nucleotides III. Pouring a 1L Phosphocellulose (PC) Column IV. Brains V. Centrifuges
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
immunofluorescence. Block in AbDil for 10'. Anti-tubulin for 20' - 30' (We use DM1alpha) Wash 4x TBST (TBST = TBS + 0.1% Triton X-100) Secondary for 20' - 30' Wash 4X TBST. Wash once with TBST + 1 ug/ml Hoechst
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