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- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
400uL
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文献和实验and reagents for use with RNA. All buffers are subjected to rigorous quality control procedures and are guaranteed RNase- and DNase-free. Preparing RNase-free Solutions DEPC Treatment Diethylpyrocarbonate (DEPC) treatment
are normalized using 18S rRNA levels. 6) The extent of target gene knockdown is expressed as a percent of mRNA remaining in cells treated with the gene-specific siRNA compared to cells treated with a negative control siRNA (Silencer Negative Control siRNA
Real-Time PCR Quantification Using Cloned Standards and Multiple Housekeeping Genes
and runs, operator errors, and the presence of PCR inhibitors (3). This will inevitably introduce noise in the experimental data. Therefore, there have been many attempts to control these errors including normalizing the data to total RNA, 18s or 28s rRNA
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