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- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
20ul
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文献和实验PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
Identifying Differential Histone Modification Sites from ChIPseq Data
Epigenetic modifications are critical to gene regulations and genome functions. Among different epigenetic modifications, it is of great interest to study the differential histone modification sites (DHMSs), which contribute to the epigenetic
Chromatin Immunoprecipitation Protocol for Histone Modification
. This has been very successful when using antibodies that detect site specific histone lysine methylation (Peters et al . 2003, Martens et al . 2005). However, for each different antibody the optimal concentration should be tested and calibrated. The stringency of washing
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