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- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
500mg
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
well.Add GTP to 1 mM and DMSO to 10% and mix well.Incubate at 37℃ for 10 min.The solution should turn turbid and viscous. 3.Centrifuge in a SS34 rotor at 18,000 rpm,37℃ for 20 min.Keep the rotor warm in an incubator afterwards. 4.Discard the supernatant
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
Day 0 and 1 Materials 1.0.5 mM ATP,0.2 mM CaCl2,1 mM PIPES,pH 6.8 at 4℃,250 ml for day 0. 2.0.5 mM ATP,0.2 mM CaCl2,70 mM KCl,150 mM PIPES,pH 7.0,1 ml. 3.16 mM ATP,0.2 mM CaCl2,5 mM DTT,5 mM lysine,10 mM glutamic acid,20 mM Tris-HCl,pH 8.0,10 ml
PEG Preparation of Plasmid DNA
in a microfuge for 5 minutes for small volumes, 10,000g in a MSE Europa or equivalent for 20 minutes for large volumes). 6) Remove the clear supernatant to a clean tube. 7) Add DNase-free RNase A (0.01 supernatant volume of 10mg per ml stock), mix
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