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- 文献和实验
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- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
50uL
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文献和实验. To each tube with the tail piece, add 0.5 ml of the following tail chop digestion buffer: 2.5 ml 1M Tris, pH 8 10.0 ml 0.5M EDTA 5.0 ml 10% SDS 31.5 ml ddI H2 O The final volume is 50 ml. To the amount needed
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
. for the duration of the harvesting process, after which we think these tails can be kept at 4℃ overnight without much trouble. To each tube with the tail piece, add 0.5 ml of the following tail chop digestion buffer: 2.5 ml 1M Tris, pH 8 10.0 ml 0.5M EDTA
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