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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
100ml
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文献和实验Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
Methods for the Detection of D-Amino-Acid Oxidase
on the activity of the samples. It was terminated by the addition of 1 ml of 10% trichloroacetic acid. In a blank, trichloroacetic acid was added to the reaction mixture before the enzyme reaction was started. The precipitate was removed by centrifugation (700 x g
Bicinchoninic Acid (BCA) Protein Assay (Smith)
with distilled water.Adjust the pH to 11.25 with 10 M NaOH. 2.Reagent B: 0.4 gm cupric sulfate (5 x hydrated)in 10 ml distilled water. 3.Standard working solution (SWR): Mix 100 volumes reagent A with 2 volumes reagent B. 4.The stock solutions are stable
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