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- OZ Biosciences
- OZB-TN31000-1mL
- 2026年02月19日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
1mL
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文献和实验Transfection of siRNA oligos to peritoneal macrophages
Use DeliverX Transfection from Panomics Modified protocol below Step 1: Make complexes Note: Do not scale up more than 3x Thaw siRNA and DeliverX reagent Dilute siRNA into buffer 1 10 uM siRNA 2.17 ul Buffer
Transient transfection into 293T cells
volume of the medium should be 3ml and 1.5ml, respectively, and decrease the amount of each reagent accordingly. 1. Plate cells the night before to give 60-70% confluence at the day of transfection. The efficiency will decrease if reached 100% confluence
Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
Reagent, TR-10 10-Liter Transient transfection • BrightLine Hemacytometer, Sigma, • Trypan Blue Solution (0.4%), Sigma, • 0.22 um GP Express Membrane Filter, Millipore IV. GENERAL CONSIDERATIONS A. Prior to Startup Since 293 cells tend
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