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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
500uL
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文献和实验Transfection of siRNA oligos to peritoneal macrophages
Use DeliverX Transfection from Panomics Modified protocol below Step 1: Make complexes Note: Do not scale up more than 3x Thaw siRNA and DeliverX reagent Dilute siRNA into buffer 1 10 uM siRNA 2.17 ul Buffer
Transient transfection into 293T cells
: optional) • Transfection reagents: i) 2M CaCl2 : 2M in H2 O, filter sterile, store at 4°C ii) x2HBS: 8.0g NaCl, 0.37g KCl, 201mg (yes! mg) Na2 HPO4 •7H2 O, 1.0g glucose, 5.0g HEPES/500ml (adjust pH to 7.05 with NaOH and filter sterile
Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
Orbital Shaker • Water Bath, VWR Scientific • 500 ml Vented Cap Erlenmymer Flask, • 125 ml Vented Cap Erlenmymer Flask, Corning product • Pro293S-CDM, 1 L (Cambrex Biosciences, Cat 12-765Q) • Penicillin-Streptomycin, Invitrogen • Transfection
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